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Protein interactions and eukaryotic protein acquisition. (A) Schematic overview of the screening strategy and identification of T. gondii KCR. (B) Co‐immunoprecipitation identification of the interaction between KCR and murine CSF2Rα input: cell lysates from HEK 293T cells co‐transfected with pcDNA3.1‐KCR and pCAGGS‐CSF2R for 24 h; IP: KCR, CSF2α or IgG: immunoprecipitation was performed using Flag‐tag mouse <t>mAb,</t> <t>His‐tag</t> mouse mAb or mouse IgG; IB: KCR or CSF2α: immunoblot analysis was performed using Flag‐tag rabbit mAb or His‐tag rabbit pAb. (C) Acquisition of KCR eukaryotic protein. Lane M: standard molecular marker for protein; lane 1: cell lysates from HEK 293T cells transfected with pcDNA3.1‐KCR for 24 h; lane 2: purified KCR eukaryotic protein. (D) Western blot analysis of KCR M: standard molecular marker for protein; lane 3: his‐tag in purified KCR was identified by His‐tag mouse mAb.
His Tag Mouse Mab, supplied by Abmart Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Protein interactions and eukaryotic protein acquisition. (A) Schematic overview of the screening strategy and identification of T. gondii KCR. (B) Co‐immunoprecipitation identification of the interaction between KCR and murine CSF2Rα input: cell lysates from HEK 293T cells co‐transfected with pcDNA3.1‐KCR and pCAGGS‐CSF2R for 24 h; IP: KCR, CSF2α or IgG: immunoprecipitation was performed using Flag‐tag mouse <t>mAb,</t> <t>His‐tag</t> mouse mAb or mouse IgG; IB: KCR or CSF2α: immunoblot analysis was performed using Flag‐tag rabbit mAb or His‐tag rabbit pAb. (C) Acquisition of KCR eukaryotic protein. Lane M: standard molecular marker for protein; lane 1: cell lysates from HEK 293T cells transfected with pcDNA3.1‐KCR for 24 h; lane 2: purified KCR eukaryotic protein. (D) Western blot analysis of KCR M: standard molecular marker for protein; lane 3: his‐tag in purified KCR was identified by His‐tag mouse mAb.
Mouse Anti His Antibody, supplied by Epizyme Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Protein interactions and eukaryotic protein acquisition. (A) Schematic overview of the screening strategy and identification of T. gondii KCR. (B) Co‐immunoprecipitation identification of the interaction between KCR and murine CSF2Rα input: cell lysates from HEK 293T cells co‐transfected with pcDNA3.1‐KCR and pCAGGS‐CSF2R for 24 h; IP: KCR, CSF2α or IgG: immunoprecipitation was performed using Flag‐tag mouse <t>mAb,</t> <t>His‐tag</t> mouse mAb or mouse IgG; IB: KCR or CSF2α: immunoblot analysis was performed using Flag‐tag rabbit mAb or His‐tag rabbit pAb. (C) Acquisition of KCR eukaryotic protein. Lane M: standard molecular marker for protein; lane 1: cell lysates from HEK 293T cells transfected with pcDNA3.1‐KCR for 24 h; lane 2: purified KCR eukaryotic protein. (D) Western blot analysis of KCR M: standard molecular marker for protein; lane 3: his‐tag in purified KCR was identified by His‐tag mouse mAb.
Mouse Anti His Tag Mab, supplied by Cowin Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sangon Biotech hrp conjugated mouse anti his antibody
Protein interactions and eukaryotic protein acquisition. (A) Schematic overview of the screening strategy and identification of T. gondii KCR. (B) Co‐immunoprecipitation identification of the interaction between KCR and murine CSF2Rα input: cell lysates from HEK 293T cells co‐transfected with pcDNA3.1‐KCR and pCAGGS‐CSF2R for 24 h; IP: KCR, CSF2α or IgG: immunoprecipitation was performed using Flag‐tag mouse <t>mAb,</t> <t>His‐tag</t> mouse mAb or mouse IgG; IB: KCR or CSF2α: immunoblot analysis was performed using Flag‐tag rabbit mAb or His‐tag rabbit pAb. (C) Acquisition of KCR eukaryotic protein. Lane M: standard molecular marker for protein; lane 1: cell lysates from HEK 293T cells transfected with pcDNA3.1‐KCR for 24 h; lane 2: purified KCR eukaryotic protein. (D) Western blot analysis of KCR M: standard molecular marker for protein; lane 3: his‐tag in purified KCR was identified by His‐tag mouse mAb.
Hrp Conjugated Mouse Anti His Antibody, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sino Biological anti his tag antibody
Protein interactions and eukaryotic protein acquisition. (A) Schematic overview of the screening strategy and identification of T. gondii KCR. (B) Co‐immunoprecipitation identification of the interaction between KCR and murine CSF2Rα input: cell lysates from HEK 293T cells co‐transfected with pcDNA3.1‐KCR and pCAGGS‐CSF2R for 24 h; IP: KCR, CSF2α or IgG: immunoprecipitation was performed using Flag‐tag mouse <t>mAb,</t> <t>His‐tag</t> mouse mAb or mouse IgG; IB: KCR or CSF2α: immunoblot analysis was performed using Flag‐tag rabbit mAb or His‐tag rabbit pAb. (C) Acquisition of KCR eukaryotic protein. Lane M: standard molecular marker for protein; lane 1: cell lysates from HEK 293T cells transfected with pcDNA3.1‐KCR for 24 h; lane 2: purified KCR eukaryotic protein. (D) Western blot analysis of KCR M: standard molecular marker for protein; lane 3: his‐tag in purified KCR was identified by His‐tag mouse mAb.
Anti His Tag Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Boster Bio mouse anti his tag mab
Protein interactions and eukaryotic protein acquisition. (A) Schematic overview of the screening strategy and identification of T. gondii KCR. (B) Co‐immunoprecipitation identification of the interaction between KCR and murine CSF2Rα input: cell lysates from HEK 293T cells co‐transfected with pcDNA3.1‐KCR and pCAGGS‐CSF2R for 24 h; IP: KCR, CSF2α or IgG: immunoprecipitation was performed using Flag‐tag mouse <t>mAb,</t> <t>His‐tag</t> mouse mAb or mouse IgG; IB: KCR or CSF2α: immunoblot analysis was performed using Flag‐tag rabbit mAb or His‐tag rabbit pAb. (C) Acquisition of KCR eukaryotic protein. Lane M: standard molecular marker for protein; lane 1: cell lysates from HEK 293T cells transfected with pcDNA3.1‐KCR for 24 h; lane 2: purified KCR eukaryotic protein. (D) Western blot analysis of KCR M: standard molecular marker for protein; lane 3: his‐tag in purified KCR was identified by His‐tag mouse mAb.
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Qiagen mouse anti his tag monoclonal hrp
Protein interactions and eukaryotic protein acquisition. (A) Schematic overview of the screening strategy and identification of T. gondii KCR. (B) Co‐immunoprecipitation identification of the interaction between KCR and murine CSF2Rα input: cell lysates from HEK 293T cells co‐transfected with pcDNA3.1‐KCR and pCAGGS‐CSF2R for 24 h; IP: KCR, CSF2α or IgG: immunoprecipitation was performed using Flag‐tag mouse <t>mAb,</t> <t>His‐tag</t> mouse mAb or mouse IgG; IB: KCR or CSF2α: immunoblot analysis was performed using Flag‐tag rabbit mAb or His‐tag rabbit pAb. (C) Acquisition of KCR eukaryotic protein. Lane M: standard molecular marker for protein; lane 1: cell lysates from HEK 293T cells transfected with pcDNA3.1‐KCR for 24 h; lane 2: purified KCR eukaryotic protein. (D) Western blot analysis of KCR M: standard molecular marker for protein; lane 3: his‐tag in purified KCR was identified by His‐tag mouse mAb.
Mouse Anti His Tag Monoclonal Hrp, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Protein interactions and eukaryotic protein acquisition. (A) Schematic overview of the screening strategy and identification of T. gondii KCR. (B) Co‐immunoprecipitation identification of the interaction between KCR and murine CSF2Rα input: cell lysates from HEK 293T cells co‐transfected with pcDNA3.1‐KCR and pCAGGS‐CSF2R for 24 h; IP: KCR, CSF2α or IgG: immunoprecipitation was performed using Flag‐tag mouse mAb, His‐tag mouse mAb or mouse IgG; IB: KCR or CSF2α: immunoblot analysis was performed using Flag‐tag rabbit mAb or His‐tag rabbit pAb. (C) Acquisition of KCR eukaryotic protein. Lane M: standard molecular marker for protein; lane 1: cell lysates from HEK 293T cells transfected with pcDNA3.1‐KCR for 24 h; lane 2: purified KCR eukaryotic protein. (D) Western blot analysis of KCR M: standard molecular marker for protein; lane 3: his‐tag in purified KCR was identified by His‐tag mouse mAb.

Journal: Transboundary and Emerging Diseases

Article Title: Toxoplasma gondii KCR is a Noncanonical Modulator of CSF2 Signaling that Targets the CSF2Rα–JAK2/STAT5 Axis

doi: 10.1155/tbed/8426765

Figure Lengend Snippet: Protein interactions and eukaryotic protein acquisition. (A) Schematic overview of the screening strategy and identification of T. gondii KCR. (B) Co‐immunoprecipitation identification of the interaction between KCR and murine CSF2Rα input: cell lysates from HEK 293T cells co‐transfected with pcDNA3.1‐KCR and pCAGGS‐CSF2R for 24 h; IP: KCR, CSF2α or IgG: immunoprecipitation was performed using Flag‐tag mouse mAb, His‐tag mouse mAb or mouse IgG; IB: KCR or CSF2α: immunoblot analysis was performed using Flag‐tag rabbit mAb or His‐tag rabbit pAb. (C) Acquisition of KCR eukaryotic protein. Lane M: standard molecular marker for protein; lane 1: cell lysates from HEK 293T cells transfected with pcDNA3.1‐KCR for 24 h; lane 2: purified KCR eukaryotic protein. (D) Western blot analysis of KCR M: standard molecular marker for protein; lane 3: his‐tag in purified KCR was identified by His‐tag mouse mAb.

Article Snippet: Flag‐tag mouse monoclonal antibody (mAb) (#M20008), His‐tag mouse mAb (# M20001 ), and mouse IgG (#B30010M) were purchased from Abmart Biotech, Inc. (Shanghai, China).

Techniques: Immunoprecipitation, Transfection, FLAG-tag, Western Blot, Marker, Purification